Fig. 5

Elevated p53 is responsible for the survival of the erythroblast (A) Volcano plot of differentially expressed coding genes (DEGs) between erythroblast from add1−/− and siblings. Vertical lines correspond to 2-fold differences; horizontal line represents a p value of 0.05. Red and blue points represent DEGs with statistical significance. The differentially expressed genes enriched in p53 signaling pathway were indicated. (B) Pathway enrichment analysis identified affected in mutants. (C) The expression levels of p53 related genes were examined by qRT-PCR. The expression levels of β-actin were used as a reference to normalize the amounts of mRNAs in each group. Significance of differences: ∗∗, p < 0.01, ∗∗∗, p < 0.001 (by Student’s t test). (D) Expression pattern of p53 related genes in add1 mutant embryos detected by WISH. The expression levels of these genes were elevated in add1 mutant embryos. (E) Detection of hemoglobin in different embryos using o-dianicidine staining. Note that there are apparent positive signals in the add1;p53 double mutants embryos at the indicated stage. (F) Dural luciferase reporter assay of ADD1 regulating the p53 promoter. Confirmation of Add1 inhibiting the promoter activity of p53. ∗∗∗, p < 0.001 (by Student’s t test).