FIGURE

Figure 6

ID

ZDB-FIG-231218-19

Publication

Liao et al., 2023 - Deletion of prolyl hydroxylase domain-containing enzyme 3 (phd3) in zebrafish facilitates hypoxia tolerance

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Figure 6

Zebrafish phd3 suppresses HIF transcriptional activation.A, luciferase activity of hypoxia-responsive element (HRE) reporter in EPC cells transfected with Myc-phd3 or Myc empty vector (control) under normoxia (21% O₂) or hypoxia (1% O₂) for 24 h. B, luciferase activity of the HRE reporter in EPC cells co-transfected with Flag-hif1αa or Flag empty vector (control) together with an increasing amount of Myc-phd3 under normoxia (21% O₂) for 24 h. C, luciferase activity of the HRE reporter in EPC cells co-transfected with Flag-hif1αb or Flag empty vector (control) together with an increasing amount of Myc-phd3 under normoxia (21% O₂) for 24 h. D, luciferase activity of the HRE reporter in EPC cells co-transfected with Flag-hif2αa or Flag empty vector (control) together with an increasing amount of Myc-phd3 under normoxia (21% O₂) for 24 h. E, luciferase activity of the HRE reporter in EPC cells co-transfected with Flag-hif2αb or Flag empty vector (control) together with an increasing amount of Myc-phd3 under normoxia (21% O₂) for 24 h. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, using two-way ANOVA analysis; data are representative of three independent experiments (mean ± SD of three technical replicates).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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