Fig. 7
Laminin α2 overexpression reduces the number of SCs in controls and csp−/− embryos. (A) Quantification of the number of SCs within a defined region of the PLLn at 48 hpf in control (average of 72.60±1.78 cells, n=15 embryos), control injected with pacta1-lama2 (average of 49.55±3.18 cells, n=11 embryos), csp−/− (average of 53.09±2.15, n=11 embryos) and csp−/− injected with pacta1-lama2 (average of 42.60±1.56 cells, n=20 embryos) (****P<0.0001; **P=0.004). (B) Quantification of the number of PH3+ SCs within a defined region of the PLLn at 48 hpf in control (average of 1.26±0.36 cells, n=15 embryos), control injected with pacta1-lama2 (average of 1.11±0.26 cells, n=9 embryos), csp−/− (average of 10.56±1.34 cells, n=9 embryos) and csp−/− injected with pacta1-lama2 (average of 4.70±1.01 cells, n=10 embryos) (ns, P>0.9999, control versus control+pacta1-lama2; ns, P=0.28, csp−/− versus csp−/−+pacta1-lama2). (C) Quantification of the percentage of PH3+ SCs relative to the total number of SCs within a defined region of the PLLn at 48 hpf in control (average of 1.68±0.38 cells, n=13 embryos), control injected with pacta1-lama2 (average of 2.36±0.57 cells, n=9 embryos), csp−/− (average of 20.08±2.83 cells, n=9 embryos) and csp−/− injected with pacta1-lama2 (average of 11.99±2.51 cells, n=10 embryos) (ns, P>0.9999; *P=0.022). (D) Quantification of the time required for control (average of 9.66±0.39, 12 cells, n=4 embryos), csp−/− (average of 86.80±4.77, ten cells, n=4 embryos) and csp−/− injected with pacta1-lama2 (average of 88.60±4.31, ten cells, n=4 embryos) SCs to successfully complete cytokinesis during migration (****P≤0.0001; ns, P=0.7828). (E) Quantification of the time required for control (average of 9.77±0.61, nine cells, n=4 embryos), csp−/− (average of 94.42±14.18, 19 cells, n=4 embryos) and csp−/− injected with pacta-lama2 (average of 100.2±10.01, 12 cells, n=4 embryos) SCs to successfully complete cytokinesis during radial sorting (****P≤0.0001; ns, P=0.1424). |