Fig. 2

SCs in csp−/− show delays in mitotic progression but exit mitosis with no significant increase in apoptosis. (A) PH3 immunolabeling in Tg(foxd3:gfp) and Tg(foxd3:gfp)/csp−/− larvae at 48 hpf and 72 hpf. Arrows indicate SCs that are GFP and PH3 positive. Scale bar: 25 µm. (B) Quantification of the number of SCs within a defined region of the PLLn at 48 and 72 hpf in control (average of 72.77±1.96 cells at 48 hpf, n=13 embryos; average of 69.60±1.61 cells at 72 hpf, n=17 embryos) and csp−/− (average of 53.67±2.50 cells at 48 hpf, n=9 embryos; average of 59.60±2.10 cells at 72 hpf, n=10 embryos) (****P≤0.0001; **P=0.0012). (C) Quantification of the number of PH3+ SCs within a defined region of the PLLn at 48 and 72 hpf in control (average of 1.26±0.33 cells at 48 hpf, n=15 embryos; average of 0.45±0.17 cells at 72 hpf, n=20 embryos) and csp−/− (average of 10.56±1.34 cells at 48 hpf, n=9 embryos; average of 0.77±0.27 cells at 72 hpf, n=9 embryos) (****P≤0.0001; ns, P=0.33). (D) Quantification of the percentage of PH3+ SCs relative to the total number of SCs within a defined region of the PLLn at 48 and 72 hpf in control (average of 1.68±0.38 cells at 48 hpf, n=13 embryos; average of 0.67±0.26 cells at 72 hpf, n=20 embryos) and csp−/− (average of 20.08±2.83 cells at 48 hpf, n=9 embryos; average of 1.25±0.46 cells at 72 hpf; n=9 embryos) (***P=0.0002; ns, P=0.19).