FIGURE

Fig. 2

ID

ZDB-FIG-231127-95

Publication

Zhong et al., 2023 - Eurycomanone stimulates bone mineralization in zebrafish larvae and promotes osteogenic differentiation of mesenchymal stem cells by upregulating AKT/GSK-3β/β-catenin signaling

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Fig. 2

The chemical structure of EN and effects of EN on skull mineralization in zebrafish larvae. (a) Chemical structure of EN. (b) Zebrafish larvae were treated with EN (0.2, 1, and 5 μM), and skull mineralization ability was determined using ARS. (c) Mineralization areaJOTr-D-22-00514R1. (d) Mineralization IOD. (e) The mRNA expression of osteoblast-related genes (ALP, RUNX2a, SP7, and OCN) on 9-dpf in zebrafish larvae. The data are shown as the means ± SD (n = 10 zebrafish larvae/group). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Con. Scale bar: 100 μm. EN, eurycomanone; ARS, alizarin red S; IOD, integrated optical density; ALP, alkaline phosphatase, RUNX2a, runt-related transcription factor 2a; SP7, Sp7 transcription factor; OCN, osteocalcin; dpf, days after fertilization; Con, Control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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