FIGURE 5

Overlap of DNA CpG promoter methylation in tet2^m/m fish with changes previously reported in human MDS specimens. (A) The percentage differences in the methylation of cytosines within the promoter regions (500 bp to +250 bp upstream and downstream of the transcription initiation site) in the HSPCs of 15-month-old tet2^m/m fish compared with those 15-month-old tet2^wt/wt fish, with the latter as the baseline. The percentage difference in the methylation status of the CpG sites in each promoter region is plotted on the x-axis and the negative logarithm of the adjusted p-value is on the y-axis. The regions with an absolute change greater than 15% and an adjusted p-value less than 0.05 are colored. The genes that were hypermethylated in the 15-month-old tet2^m/m fish are colored in orange and those that were hypomethylated are colored in blue. (B) Overlap of differentially methylated promoters identified in Figure 5A with those identified in human MDS specimens. Common genes are highlighted—genes in orange are hypermethylated in the HSPCs of 15-month-old tet2^m/m fish and those in blue are hypomethylated in the HSPCs of 15-month-old tet2^m/m fish.