FIGURE

FIGURE 1

ID

ZDB-FIG-231108-22

Publication

Neelamraju et al., 2023 - Depletion of tet2 results in age-dependent changes in DNA methylation and gene expression in a zebrafish model of myelodysplastic syndrome

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FIGURE 1

Schematic overview of studies of DNA methylation and RNA expression by zebrafish kidney marrow cells. The site of hematopoiesis in the adult zebrafish is in the head kidney (upper left of the diagram). Head kidney marrow cells from 4- and 15-month-old zebrafish were isolated and the progenitor cells were sorted by fluorescence-activated cell sorting based on their forward and side light scatter properties, as previously described (7). The sorted progenitor cells were then processed for DNA extraction using enhanced reduced representation of bisulfite sequencing to assess cytosine methylation. RNA was also extracted from an aliquot of the sorted progenitor cells for poly(A)+ RNA sequencing, as described in the Methods section. tet2, Ten-eleven translocation 2; ERRBS, Enhanced Reduced Representation of Bisulfite sequencing; Ey, erythrocytes; Ly, lymphocytes; Pr, progenitors; My, myelomonocytes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Hematol