Figure 4

Excess carbohydrates damaged zebrafish liver health and increased inflammation-related genes in zebrafish liver and ZFL (means ± SEM, n = 6). C0, C10, C20, C30, C40, and C50 represent diets in which dextrin levels are 0%, 10%, 20%, 30%, 40%, and 50%, respectively. L represents a linear trend, Q represents a quadratic trend, C represents a cubic trend, and # represents the significant of the corresponding regressions. HG represents high glucose. (a) Representative images of the liver H&E staining, scale bar, 50 μm, (b) the relative health status of the liver was scored according to the degree of vacuolization (1–2: severe damage, 3–4: moderate, 5–6: mild, 7–8: slight, 9–10: normal), (c–e) inflammation-related genes' expression in zebrafish liver, and (f) inflammation-related genes' expression in ZFL. Labeled means without a common letter differ among C0, C10, C20, C30, C40, and C50, P < 0.05 (one-way ANOVA, Duncan's post hoc test).  ^∗P < 0.05,  ^{∗ ∗ ∗}P < 0.005 (two-tailed independent t-test).