FIGURE

FIGURE 5

ID

ZDB-FIG-231020-5

Publication

Wei et al., 2023 - cxcl12a plays an essential role in pharyngeal cartilage development

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FIGURE 5

cxcl12a mutants exhibit reduced pharyngeal pouch cells. (A) Expression levels of nkx2.3 and cv2 expression were assayed in wild-type or cxcl12a mutants post-injection with either control MO or cxcl12b MO. (B–C) Confocal microscopy elucidates images showing pharyngeal pouch cellular architecture in wild-type and cxcl12a, or combined cxcl12a and cxcl12b knockdown embryos within a Tg(nkx2.3:mCherry)(B) or Tg(sox17:GFP)(C) background at 36 hpf. Scale bar, 50 μm. (D) Quantification of mCherry-positive cells within the Tg(nkx2.3-mCherry) transgenic line was executed from a sample size of nine embryos. Error bars are indicative of standard deviation (SD). Statistical significance denoted by *, p < 0.05; **, p < 0.01, as gauged by Student’s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol