Figure 6

Bmp4 promotes the expression of antiviral genes. (A,B) Expression of EPC ifn and EPC mx mRNA after transfection with bmp4 (2 μg) or empty vector (2 μg) in EPC cells. The cells were collected 24 h or 36 h post transfection. (C,D) Expression of EPC ifn and EPC mx mRNA after transfection with bmp4 (2 μg) or empty vector (2 μg) in EPC cells for 24 h, followed by infection with GCRV for another 24 h or 48 h. (E,F) Expression of ifnφ1 and mxa mRNA from WT or bmp4^−/− zebrafish larvae challenged with GCRV for 24 h or 36 h. (G,H) Expression of ifnφ1 and mxa mRNA in the liver and spleen from WT or bmp4^−/− adult zebrafish challenged by injecting i.p. with 40 µL of GCRV (2.5 × 10⁷ TCID₅₀/mL) for 36 h. The expression of zebrafish actb1 or EPC actin was used as an internal control for the qRT-PCR. Data were from three independent experiments and were analyzed using Student’s t-test (two-tailed) for comparison of two groups. All data are presented as mean ± SD (** p < 0.01, *** p < 0.001, **** p < 0.0001).