Figure 3

The morphology of bipolar cell ribbon synapses in the retinal IPL was altered in older-aged zebrafish. (A,B) Transverse retinal sections from middle-aged ((A), left panels; n = 2~4 sections in four retinas from two fish) and old-aged ((B), right panels; n = 2~4 sections in four retinas from two fish) zebrafish were double immunostained with fluorescently labeled antibodies specific for the rod bipolar cell marker PKCα (cyan; top panels) or ribeye a (red; middle panels); also shown is the overlay of PKCα and ribeye a labeling (merge; bottom). Maximal intensity projections are shown, and the relative positions of the INL and IPL are indicated. Scale bar, 20 µm. PKCα, protein kinase C alpha; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Larger (arrow) and smaller (arrowhead) are indicated in PKCα ribbon terminal morphologies in MA and OA. # Denotes the pear-shaped soma characteristics of Mb1 rod bipolar cells with larger terminals.