Figure 2

The retinal bipolar cells isolated from older-aged zebrafish exhibit changes in the numbers and length of their synaptic ribbons. (A,B) Representative two-dimensional projections of the bipolar cell synaptic terminals from middle-aged (MA; panel (A); n = 19 cells in seven animals) and older-aged (OA; panel (B); n = 19 cells in seven animals) zebrafish in which the synaptic ribbons were labeled by voltage clamping with a whole-cell pipette, whose internal solution contained fluorescent TAMRA-RBP peptides and was visualized by confocal microscopy. Scale bar, 2 µm. (C,D) Imaris-generated three-dimensional (3D)-reconstruction of zebrafish bipolar cells whose synaptic ribbons were labeled with TAMRA-RBP (red) and visualized as described above. Scale bar, 5 µm. (E,F) The average number of ribbons (C) and the ribbon lengths (D) contained by zebrafish bipolar neurons isolated from MA (A) or older-aged (B) zebrafish as described in Section 2.