FIGURE

Fig. 4.

ID

ZDB-FIG-231007-4

Publication

Bui et al., 2023 - Apollo-NADP⁺ reveals in vivo adaptation of NADPH/NADP⁺ metabolism in electrically activated pancreatic β cells

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Fig. 4.

Selective inhibition of NADP⁺ reduction pathways in unstressed and stressed INS1E pancreatic β cells.

(A) INS1E β cells transfected with Apollo-NADP⁺. Top: Fluorescence intensity image of Apollo-NADP⁺ expressed in INS1E β cells. Bottom: Fluorescence anisotropy images of Apollo-NADP⁺ expressing INS1E β cells stimulated with glucose. (B) Glucose-stimulated NADP⁺ reduction of INS1E β cells transfected with Apollo-NADP⁺. UK (50 μM) was used to inhibit pyruvate cycling, 6AN (5 μM) was used to inhibit the pentose phosphate pathway, and MTX (5 μM) was used to inhibit folate cycling. Chemical inhibitors were added 1 hour before imaging. (C) Glucose-stimulated NADP⁺ reduction of INS1E β cells transfected with Apollo-NADP⁺. Stress was induced by 8 hours of stress treatment with 100 μM TBM. n = 3 to 6 replicates. *P < 0.05 and ****P < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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