Fig. 4

Effect of Omd on the spontaneous development of subchondral bone sclerosis. a Histomorphometry of the subchondral bone on Safranin-O Fast Green of the knee joint of male mice at 8 and 16 months was performed with QuPath on the lateral and medial plateaus of the tibia separately. At 8 months: n = 8 for each genotype; at 16 months: n = 7 for the KO, n = 7 for the WT, and n = 9 for the UP. The data were plotted as a box plot showing all points. Two-way ANOVA was performed with differences being considered significant at P values <0.05 (*P < 0.05, **P ≤ 0.01). b Representative picture of the Safranin-O Fast Green of the knee joint of male mice showing the subchondral bone area for the lateral and medial plateaus of the tibia separately. Scale bar = 100 µm. c µCT of the subchondral bone of the tibia of the mice at 16 months. The pink asterisk indicates the lateral plateau and the blue asterisk indicates the medial plateau