Figure 3

Figure 3. atm mutation is well tolerated in zebrafish embryos. (A) The percentage of mating success calculated from 5 individual pairs over 6 independent matings for WT and atm^−/− mutants. Each dot indicates the average success of each mating pair based on the production of any embryos. (B) The number of embryos generated for paired mating of WT and atm^−/− adults. Each dot indicated a different mating. (C) Images of WT and atm^−/− mutants after squeezing and at 4 h post-fertilization (hpf) showing fertilized, unfertilized and dead embryos. (D) Stack bar indicates dead, fertilized, and unfertilized as categorized in panel C for individual mating pairs of WT and atm^−/− mutants. Summary stack bars show the average of each phenotype for WT and atm^−/− mutants. (E) Survival curve of WT and atm^−/− mutants from 1 to 5 dpf indicates no significant differences between fertilized embryos of WT and atm^−/− mutants. (F) Representative images of live WT and atm^−/− mutants from 1 to 5 dpf show no observable morphological differences are detected. Values are expressed as the mean ± SD and were compared by unpaired t-test. Significant differences are indicated as follows: ns p > 0.05 (non-significant), * p < 0.05.