Figure 5

Figure 5. atm mutation enhanced the small liver phenotype and larval death in Tg (fabp10a:hUHRF1-EGFP) larvae. (A) Liver size of hUHRF1 and atm^−/−; hUHRF1 larvae compared to non-transgenic WT and atm^−/− siblings at 5 and 7 dpf shows that atm mutation synergizes with hUHRF1 overexpression to decrease in liver size by 7 dpf. Experiments was performed in 2 biological replicates; each dot represents 1 liver. The middle line in the box plot represents the median, with the whiskers representing the range of the values for each condition. B. Survival curve of WT, atm^−/−, hUHRF1 and atm^−/−; hUHRF1 larvae from 5 to 20 dpf. (C,D) hUHRF1 with WT atm and atm^−/−;hUHRF1 larvae were separated into 2 groups at 7 dpf based the liver size being above or below the median of the size measured in atm^−/−; hUHRF1 larvae. Dots indicate number of livers and were assessed for survival at 7 and 20 dpf (D). Values are expressed as the mean ± SD and were compared by unpaired t-test (A,C,D) or by long-rank test (B). Significant differences are indicated as follows: n.s. indicates p > 0.05 (non-significant), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as determined by a 2-way ANOVA.