Figure 5.

Pharmacological blockade of MR results in a reduced macrophage migration toward a wound. (A) Number of mpeg+ macrophages in the whole body of zebrafish at 52 hpf after incubation with either 1.25 μM or 12.5 μM eplerenone for 50 hours (n = 8-12). (B) Number of mpeg+ macrophages that have migrated toward the wound site (200 µm from point of amputation) at 4 hpa in zebrafish exposed to either vehicle or the MR antagonist eplerenone (1.25 μM) for 50 hours (n = 16). (C) Number of macrophages that have migrated toward the wound site, at 4 hpa, in zebrafish exposed to either eplerenone (1.25 μM) or the GR antagonist mifepristone (RU486 (1.25 μM) (n = 12-21). (D) Representative images of Tg(mpeg:mCherry) zebrafish at 48 hpf, treated with either vehicle, eplerenone (1.25 µM) or mifepristone (1.25 µM) for 6 hours. Bars show mean ± SEM of data pooled from three different experiments (A-C; each data point representing a single larva). Data were analyzed using a 1-way ANOVA (A,C) or a t-test (B). Statistical significance is indicated by *P ≤ .05.