Fig. 1.

Tango2 deficiency results in growth defects and lethality. (A) Schematics depicting the position of tango2 alleles (tango2^bwg210 and tango2^bwg211) with reference to both transcripts in zebrafish. tango2 mutations resulted in insertions and frameshift mutations in different alleles. (B) Sanger sequencing for control and tango2 alleles depicting mutations in tango2^bwg210 (c.226_227ins7) and tango2^bwg211 (c.226_227ins26). (C) Western blot analysis of proteins from control and tango2 mutant fish (1 month of age). (D) Lateral view of control and tango2 mutant larval zebrafish showing no phenotypic differences. Images are representative of n=6 larval fish. Scale bar: 0.5 mm. (E) Kaplan–Meier survival curve of control (+/+^HT) and tango2 mutants (−/−^HT) obtained from heterozygous parents, and control (+/+^M) and tango2 mutants (−/−^M) obtained from wild-type and mutant parents, respectively. n=360. (F) Body weight analysis of control (+/+ and +/−) and tango2 mutants (−/−) at 1 month. n=60. Data are presented as mean±s.e.m. Unpaired two-tailed t-test, parametric; ns, not significant; *P<0.05.