FIGURE

FIGURE 4

ID

ZDB-FIG-230916-252

Publication

Maili et al., 2023 - Disruption of fos causes craniofacial anomalies in developing zebrafish

Other Figures

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FIGURE 4

Perturbation of fos causes bone, cartilage, and tooth abnormalities. (A–D) Fos crispants (16/19) and morphants (61/62) show abnormally shaped jaw cartilages, including a smaller ethmoid plate, Meckel’s and palatoquadrate cartilages, missing basibranchial cartilages (B–C’) red arrows) and missing pharyngeal teeth (red asterisks). (E–H) Only 24% of fos crispant and morphant zebrafish showed mineralization at the fifth ceratobranchial arch (F, G), white arrows) compared to 100% of the UIC/MM (E and H). Tooth number was reduced, and tooth shape/size was altered in fos crispants and morphants compared to UIC/MM (E–H), white asterisks). UIC, uninjected control; F0, crispant; MO, morpholino; MM, mismatch.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-fosab + CRISPR2-fosab WT + MO2-fosab
Knockdown Reagents:	CRISPR1-fosab CRISPR2-fosab MO2-fosab
Observed In:	basibranchial branchiostegal ray ossification ceratobranchial 5 bone bone mineralization ceratobranchial 5 bone ossification ceratobranchial 5 tooth cleithrum ethmoid cartilage Meckel's cartilage neurocranial trabecula opercle otolith palatoquadrate cartilage parachordal cartilage parasphenoid ossification tooth 3V tooth 4V tooth 5V
Stage:	Day 5

Phenotype Detail

Acknowledgments

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