Social maintenance is modulated at a lower acute stress intensity than social approach. A, Time spent in the white zone (%) with the visual divider present (presocial) and while the social cue is visible (social) and the difference (δ) for each LD group. B, Correlation between the time in the white zone δ (%) and the number of LD presented. C, The number of fish (%) arrived in the interaction zone for each LD group following the removal of the divider. D, Example traces from above of a 0 LD and an 18 LD fish during the social phase and a 25-s example of the fish’ movement in the interaction zone on the L-R axis over time demonstrating the frequency of social interactions. E, Correlation between the time spent in the interaction zone (%) and the number of LD presented. F, Correlation between the number of interactions performed and number of LD fish were exposed to. G, Covariance coefficient matrix for each behavior categorized into endocrinological measures, escape behaviors, social approach and social maintenance. Covariance was measured across all treatment groups. H, Social approach and social maintenance measures normalized to basal for each LD intensity. Extended Data Figures 4-1, 4–2, and 4-3 are available to support this figure. Error bars denote the mean ± 1 standard deviation (SD); significant post hoc comparisons and slope significance, ns P > 0.05, *P < 0.05 ,**P < 0.01; ***P < 0.001. Abbreviations: LD, looming dot.
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