FIGURE
Fig. 7
- ID
- ZDB-FIG-230829-25
- Publication
- George et al., 2022 - Zebrafish model of RERE syndrome recapitulates key ophthalmic defects that are rescued by small molecule inhibitor of shh signaling
- Other Figures
- All Figure Page
- Back to All Figure Page
Fig. 7
Morpholino-mediated knockdown of rerea causes coloboma. Increasing concentrations of rerea morpholino was used for knocking down rerea expression (A). Severity of coloboma is denoted by: CI-mild, CII-moderate, CIII-severe. Zebrafish embryos injected with rerea morpholino consistently exhibited coloboma at 48 hpf compared with embryos injected with control morpholino (B). Representative whole mount in situ hybridization staining with pax2a in control and rerea morpholino injected embryos 24 hpf (n ~20 embryos each) (C). WT human RERE mRNA rescues coloboma in rerea morphant fish embryos (D), this rescue was observed to be statistically significant (P < .05) for 20, 40, and 80 pg of mRNA injected, but not for 10 pg. Equal amounts of the human RERE WT and mutant (c.3466 G > A, p.Gly1156Arg) mRNA (40 pg) can rescue coloboma in rerea morphants (P < .05), but the mutant mRNA is not as efficient as WT mRNA (P < .05, E). Student's t test was used to determine level of significance and “*” denotes significant difference. NS, not significant
|
Expression Data
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage: | Prim-5 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | Prim-5 to Long-pec |
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Dev. Dyn.