|
Treponema pallidum (Tp) infection decreases the numbers of microglia in Tg(mpeg1: EGFP) zebrafish embryos. The number of microglia in Tp-treated Tg(mpeg1: EGFP) transgenic zebrafish embryos (Tp group) and untreated Tg(mpeg1: EGFP) transgenic zebrafish embryos (Control group) was counted at 2, 4, and 6 days postinfection (dpi). (A) Scheme of the experimental set up. Live one-cell stage Tg (mpeg1: EGFP) transgenic zebrafish embryos were exposed to either 0.1 μL PBS (Control group) or 0.1 μL of 109/mL Tp for 2, 4, and 6 days and then imaged with a confocal microscope. The region of interest is framed in red. (B) Tg (mpeg1: EGFP) transgenic zebrafish embryos injected with Tp revealed no developmental defect at 4dpi. Scale bar = 1 mm. (C) Bright-field image and Confocal images of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 2 dpi. Scale bar = 100 μm. (D) The number of microglia of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 2 dpi. (E) Bright-field image and Confocal images of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 4 dpi. Scale bar = 100 μm. (F) The number of microglia of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 4 dpi. (G) Bright-field image and Confocal images of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 6 dpi. Scale bar = 100 μm. (H) The number of microglia of Tg (mpeg1: EGFP) transgenic zebrafish embryos at 6 dpi. The data are shown as the mean ± SD, *P <0.05, n = 8.
|
