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The interaction of NF-ĸB and PPARγ mediates the effect of Bmp8a on adipogenesis. a, c After induction of adipogenic differentiation, the downregulated (a) and upregulated (c) KEGG pathway in overexpression zebrafish bmp8a 3T3-L1 cells. b, d After induction of adipogenic differentiation, the downregulated (b) and upregulated (d) KEGG pathway in overexpression mouse Bmp8a 3T3-L1 cells. e, f Immunoblot analysis and quantification of p-IKKα/β and p-p65 in Mock, LV-ZsGreen1, and LV-bmp8a 3T3-L1 cells (n = 3). g, h Immunoblot analysis and quantification of p-IKKα/β and p-p65 in Mock, LV-ZsGreen1, and LV-Bmp8a 3T3-L1cells. Protein expression levels were quantified by ImageJ software and normalized to total protein (n = 3). i Co-immunoprecipitation and immunoblot analysis of co-transfected with PPARγ and p65 (n = 3). j Schematic drawing of predicted PPRE site in Fabp4 promoter region. k Schematic drawing of WT and PPRE site mutation Luc-report plasmids. l, m Quantification of the activity of Fabp4-promoter (l) and Fabp4-promoter-ΔPPRE (m) luciferase reporters in mouse HEK293T cells transfected with Vector, pCMV-Pparγ, or co-transfected pCMV-Pparγ and pCMV-p65, respectively. Renilla luciferase was used as the internal control (n = 3). Data were from three independent experiments and were analyzed by One-way ANOVA and were presented as mean ± SD (ns not significant, **p < 0.01, ***p < 0.001).
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