FIGURE

Fig. 1

ID

ZDB-FIG-230717-103

Publication

Park et al., 2023 - Label-free adaptive optics single-molecule localization microscopy for whole zebrafish

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Fig. 1

Experimental setup.

a Simplified layout of the experimental setup composed of the CLASS microscope (yellow box) and SMLM (cyan box). OL objective lens, GV two-axis galvanometer mirror, DG diffraction grating, BS1-4 beam splitters, DM1-2 dichroic mirrors, PBS1-2 polarizing beam splitters, SLM spatial light modulator, and gray rectangles without labels: mirrors. b Aberration correction map whose radius is 1.2/λ in spatial frequency where λ is the emission peak wavelength of Alexa Fluor 647. c, d Single-frame raw images of single-molecule PSFs simultaneously recorded without (c) and with (d) AO, respectively. Images are normalized with respect to AO on. Insets show ensemble-averaged normalized PSFs of the first 10,000 frames. Scale bars indicate 2.5 μm and 500 nm (insets). Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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