FIGURE

Fig. 6

ID

ZDB-FIG-230714-20

Publication

Watson et al., 2023 - VGLL2-NCOA2 leverages developmental programs for pediatric sarcomagenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

VGLL2-NCOA2 is transforming in mouse myoblast allograft models

(A) C2C12 mouse myoblasts transfected with human VGLL2-NCOA2 express the VGLL2-NCOA2 fusion protein 24 h post transfection and stably after passaging and selection in growth medium with G418 compared with a C2C12-pcDNA3.1 empty control.

(B) Allograft leg volume of C2C12-pcDNA3.1 control and C2C12-VGLL2NCOA2 after intramuscular injection into the leg of SCID mice. C2C12-pcDNA3.1 control was injected in n = 10 allografts and C2C12-VGLL2NCOA2 was injected in n = 6 allografts. The error bars represent the mean leg volume ± SEM. Error bars are not shown if it is within the boundaries of the symbol. Leg volume at time points were compared using a Mann-Whitney U test corrected for multiple comparisons using the Benjamini, Krieger, and Yekutieli method. *p < 0.0005. Every time point after zero is statistically significant.

(C) Allograft leg volume of C2C12-pcDNA3.1 control and C2C12-VGLL2NCOA2 after intramuscular injection into the leg of Swiss Nude mice. C2C12-pcDNA3.1 was injected in n = 10 allografts and C2C12-VGLL2NCOA2 was injected in n = 6 allografts. The error bars represent the mean leg volume ± SEM. Error bars are not shown if they are within the boundaries of the symbol. Tumor volume at time points were compared using a Mann-Whitney U test corrected for multiple comparisons using the Benjamini, Krieger, and Yekutieli method. *p < 0.0005. Every time point after zero is statistically significant.

(D) Representative hematoxylin-eosin saffron (HES) staining from a C2C12-pcDNA3.1 control allograft and a C2C12-VGLL2NCOA2 tumor allograft. Scale bars, 50 µm.

(E) Representative immunohistochemistry for Desmin, Myogenin, and Ki67 for C2C12-pcDNA3.1 control allograft and a C2C12-VGLL2NCOA2 tumor allograft. Scale bars, 50 µm.

(F) Arf6 mRNA levels from RNA-seq of allografts from Swiss Nude mice, including two C2C12-pcDNA3.1 controls and six C2C12-VGLL2NCOA2 tumors. Error bars represent mean ± SD. Arf6 mRNA expression was compared using a Welch’s t test.

(G) Representative immunohistochemistry for Arf6 protein levels in C2C12-pcDNA3.1 controls and C2C12-VGLL2NCOA2 tumors. Eleven C2C12-pcDNA3.1 controls and six C2C12-VGLL2NCOA2 tumor samples were analyzed. Results are presented in Table S4. Scale bars, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.