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Wdr5-mediated H3K4me3 promotes differentiated cell survival partially by upregulating the expression of xiap-like gene a Graphs showing H3K4me3 and RNA peaks at 5 anti-apoptotic gene loci (zgc:171740, chm, ptmab, crx and selenow2a) in WT and wdr5−/− mutant embryos at 3 dpf. Both H3K4me3 and RNA peaks at these 5 gene loci were decreased in wdr5−/− mutant embryos. b Amino acid sequence alignment between zebrafish Zgc:171740 (Danio rerio NP_001103196), human XIAP (Homo sapiens NP_001158) and Xiap (Mus musculus NP_001288568). c WISH of zgc:171740 in WT and wdr5−/− embryos at 3 dpf. d Diagram showing the gRNA targeting site and 10-bp insertion (in red) in the exon 3 of zgc:171740 mutant, which results in a premature stop codon (PTC) at 79 aa. ATG: translation start codon. e Western blots of p53, Wdr5 and β-Actin in WT and zgc:171740−/− mutant embryos at 3 dpf. f, g Cryosections of WT and zgc:171740−/− mutant embryos at 7 dpf were analyzed by TUNEL assay (in red) and the nuclear was stained with DAPI (in blue) (f). Framed area was magnified in bottom panel. I: intestine. Scale bar: 40 μm. Statistical analysis on the percentages of apoptotic cells in the intestine between WT and zgc:171740−/− mutant was in (g). h Model of the functions of Wdr5 in digestive organogenesis. Each experiment was repeated for three times with similar results and a representative was showed here. n indicates the number of zebrafish embryos in each group. Data are mean±S.D. Two-tailed t-test was applied for each individual comparison (***p < 0.001; n.s no significance).
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