Fig 4

Zebrafish Retina TH levels are affected by age and ethanol withdrawal.

Normalized retinal TH levels from zebrafish exposed to 1.5% ethanol (black bars) or water (control, grey bars) with or without caffeine (0 mg/L; 25 mg/L, 100 mg/L). (A) TH levels assessed immediately after exposure. Fish exposed at 50–59 dpf and 70–99 dpf had significantly higher retinal TH levels relative to 140–149 dpf (p<0.008; asterisk). (B) TH levels of retinal homogenates revealed significantly greater TH levels after the immediate post-exposure interval, compared to fish sacrificed after the short or long post-exposure interval (p<0.004; asterisk). The boxplots show the minimum, first quartile, median, third quartile, and maximum values for each measure after outliers were removed. Exposure ages (dpf) are indicated at the top of the bar graphs. For retina TH levels, there were 4–6 independent replicates per treatment condition (each ethanol/caffeine dose) for a total of 35 subjects. ANOVA tables and multiple comparison results for this data can be found in S1 Table, panels (I1, I2) Retinal TH levels across pre-exposure age; (J1, J2) Retinal TH levels across time of sacrifice.