Fig. 3

a, RNA isolated from wild-type (WT), habp4^-/- and transgenic habp4^-/- embryos expressing Habp4 (habp4^-/-, habp4^tg) (WT: n = 40; habp4^-/-: n = 40; habp4^-/-, habp4^tg: n = 12). b, Representative polysome profiles from WT and habp4^-/- embryos at 1 hour post-fertilization (hpf). A260, absorbance at 260 nm. c, Quantification of monosomes and polysomes from 1 hpf embryos (WT: n = 11; habp4^-/-: n = 10). d, Mendelian ratio analysis of fin-clipped adult fish. Adult dap^-/-, dap1b^-/- and WT fish are expected at 6.25% from a dap^+/-, dap1b^+/- incross (n = 9 independent crosses; heterozygous combinations are not shown; total number of fish genotyped: 1029). e, Representative polysome profiles from 1 hpf WT and dap^-/-, dap1b^-/- embryos. f, Quantification of polysome-to-monosome ratios of 1 hpf embryos derived from WT, dap^-/-, dap1b^-/-, and transgenic dap^-/-, dap1b^-/- expressing Dap or Dap1b (WT, 1 hpf: n = 18; dap^-/-, dap1b^-/-: n = 19; dap^-/-, dap1b^-/-, dap^tg: n = 7; dap^-/-, dap1b^-/-, dap1b^tg: n = 10; WT, 2 hpf: n = 21; WT, 3 hpf: n = 5). g, Scheme of the percentage of WT and dap^-/-, dap1b^-/-, habp4^-/- (triple KO) embryos that developed into larvae (Extended Data Fig. 8g-j). h, Quantification of monosomes and polysomes from 1 hpf embryos (n = 6 per genotype; representative profile in Supplementary Fig. 1j). i-j, Quantification of protein synthesis rates normalized to Rpl3 (i) or alpha-Tubulin (j) (n = 6 per genotype; Supplementary Fig. 1l; for uncropped images of membranes see Supplementary Fig. 2c). For a, c, f and h-j, n are biologically independent samples. In a, c, d, f and h-j, data are scatter dot plots with mean ± standard deviation (SD); ns, not significant. Statistical analysis was performed with Kruskal-Wallis followed by Dunn’s two-sided tests (a, f), two-sided Mann-Whitney (c, d, h), and one-way ANOVA followed by one-sided Tukey’s multiple comparisons test (i, j).