PUBLICATION

A molecular network of conserved factors keeps ribosomes dormant in the egg

Authors
Leesch, F., Lorenzo-Orts, L., Pribitzer, C., Grishkovskaya, I., Roehsner, J., Chugunova, A., Matzinger, M., Roitinger, E., Bela?i?, K., Kandolf, S., Lin, T.Y., Mechtler, K., Meinhart, A., Haselbach, D., Pauli, A.
ID
ZDB-PUB-230119-4
Date
2023
Source
Nature   613(7945): 712-720 (Journal)
Registered Authors
Chugunova, Anastasia, Leesch, Katrin Frienderike, Lorenzo-Orts, Laura, Pauli, Andrea, Pribitzer, Carina
Keywords
none
MeSH Terms
  • Peptides/metabolism
  • Xenopus laevis/embryology
  • Zebrafish Proteins*
  • Zebrafish/embryology
  • Zebrafish/metabolism
  • Conserved Sequence*
  • Ribosomes*/metabolism
  • Animals
  • Embryonic Structures
  • Cryoelectron Microscopy/methods
  • Protein Biosynthesis*
  • Embryonic Development
  • Mass Spectrometry
  • Female
  • Xenopus Proteins*
  • Ovum*/metabolism
  • Evolution, Molecular*
(all 17)
PubMed
36653451 Full text @ Nature
Abstract
Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed1-4. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
vbc17TgTransgenic Insertion
    vbc18TgTransgenic Insertion
      vbc19TgTransgenic Insertion
        vbc20
          		Small Deletion
          vbc21
            		Small Deletion
            vbc22
              		Small Deletion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              dapCRISPR1-dapCRISPR
              dapCRISPR2-dapCRISPR
              dap1bCRISPR1-dap1bCRISPR
              dap1bCRISPR2-dap1bCRISPR
              zgc:103482CRISPR1-zgc:103482CRISPR
              zgc:103482CRISPR2-zgc:103482CRISPR
              1 - 6 of 6
              Show
              Fish
              No data available
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              sfGFPEFGsfGFP
              1 - 2 of 2
              Show
              Mapping
              No data available
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              Citation
              Leesch, F., Lorenzo-Orts, L., Pribitzer, C., Grishkovskaya, I., Roehsner, J., Chugunova, A., Matzinger, M., Roitinger, E., Bela?i?, K., Kandolf, S., Lin, T.Y., Mechtler, K., Meinhart, A., Haselbach, D., Pauli, A. (2023) A molecular network of conserved factors keeps ribosomes dormant in the egg. Nature. 613(7945):712-720.