Fig. 1
- ID
- ZDB-FIG-230703-26
- Publication
- Fetsko et al., 2022 - Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis
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Fig. 1. Zebrafish uw112 mutants express glut1b:mCherry in the absence of CNS angiogenesis. (A, B) Representative confocal microscopy images showing the glut1b:mCherry and plvap:EGFP labeled blood vessels in uw112 mutants in comparison to WT embryos and gpr124 mutants. Images are dorsal views of the head (anterior left) in live embryos at 2 and 3 dpf (A and B, respectively). At 2 dpf, uw112 and gpr124 mutants show a significant lack of central arteries (CtAs) as compared to WT (A). At 3 dpf, a distinct lack of blood vessels within the brain persists in both mutants (B). At both time points, uw112 mutants express glut1b:mCherry in the primordial hindbrain channels (PHBCs) (white arrows) while gpr124 mutants do not. Scale bars are 100 μm. (C) Quantification of the number of CtAs at 2 dpf (n = 3). (D) Quantification of the vasculature within the brain parenchyma at 3 dpf. For each group (n = 3), the blood vessels labeled with mCherry and those labeled with EGFP were quantified separately from the same fish. Data in both bar graphs (C, D) are presented as means ± standard error of the mean (SEM) (∗∗p < 0.01; ∗∗∗p < 0.001; ns = not significant). |
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| Stage Range: | Long-pec to Protruding-mouth |
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| Stage Range: | Long-pec to Protruding-mouth |
Reprinted from Developmental Biology, 494, Fetsko, A.R., Sebo, D.J., Taylor, M.R., Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis, 46-59, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.