FIGURE 4

Photoreceptor outer segment is affected in rpgra^−/− zebrafish. Retinal cryosections from WT and rpgra^−/− zebrafish were labeled rods (A), red cones (B), green cones (C), blue cones (D), and UV cones (E), with specific antibodies at the ages of 1, 3, and 6 months. The yellow lines indicate the thickness of the outer segment layer of the photoreceptor; the white arrows indicate the mistrafficked Opn1lw1 protein. RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; INL, inner nuclear layer. Scale bars, 50 μm. The statistical data are presented in (F) for rods and (G–J) for cones. At least three images from three eyes of each group were quantified and analyzed using a two-tailed Student’s t-test. The results are shown as mean ± SD. **, p < 0.01; ***, p < 0.001.