FIGURE 1

Generation of the rpgra mutant zebrafish. (A) The 13 exons of zebrafish rpgra are shown with the left and right arms of the TALEN binding sequences underlined. TALEN-L and TALEN-R, the left and right arms of the TALENs binding sequences; the NsiI restriction site in the spacer region is used for mutation detection. (B) Sequencing of the c.1675_1678delinsTAAGATTGCTTGATGATTGAG rpgra mutation in homozygous zebrafish. The DNA base change was indicated with a box. (C)rpgra mRNA levels in 2- and 6-month-old WT and rpgra^−/− zebrafish eyes detected by quantitative PCR. Actb1 served as endogenous control. The result was shown as mean ± SD. **, 0.001 < p < 0.01; ***, p < 0.001. (D) Rpgra protein levels of WT and rpgra^−/− zebrafish at 2mpf were revealed by Western blot using the anti-RPGR antibody.