Figure 3

Swimming exercise maintains mitochondrial dynamic balance in diet-induced NAFLD zebrafish livers. (A) Representative TEM of zebrafish liver showing visible LDs in fish fed a high-fat diet. TEM micrographs were used to determine (B) mitochondrial numbers at 10000× magnification, (C) mitochondrial diameter at 40000× magnification, (D) mitochondrial length at 40000× magnification, and (E) mitochondrial area at 40000× magnification. (n=2) (F) Western blots of DRP1, OPA1, and MFN2. (n=6) The protein expression of (G) DRP1, (H) OPA1, and (I) MFN2 according to densitometry analysis. *, p < 0.05, **, p < 0.01, ***, p < 0.001. Data represent the mean, and error bars represent SEM. Scale bar on TEM 10000× micrographs, 2 μm; scale bar on TEM 40000× micrographs, 0.5 μm. NAFLD, non-alcoholic fatty liver disease; N, normal diet; H, high fat diet; HE, high-fat diet plus exercise; M, mitochondria; LD, lipid droplet. ns, not significant.