inpp5ka and inpp5kb mRNAs differ in expression levels in zebrafish larvae. A Gene expression determined by qPCR. inpp5ka is more highly expressed in whole body lysates through 5 dpf. B Larval tissues were excised from the eye, head, and tail for localized gene expression analysis excluding the area of the trunk around the yolk. C, D Expression for both inpp5ka (C) and inpp5kb (D) is low in the tail and increases in the eyes and brain. By 5 dpf, both are most highly expressed in the eyes. Results from the whole body from (A) are shown as reference. E, Finpp5ka (E) maintains higher expression levels than inpp5kb (F) in the tail/muscle and head at 30 dpf and in adult fish. Both genes are expressed at very high levels in the eyes. Values are averages ± SEM. 2-way ANOVA results for tissue and developmental timepoint (time) are also listed. *p < 0.05, **p < 0.01, ***p < 0.001
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