Figure 2

chd7^−/− and phox2ba morpholino (MO) −injected larvae have lower heart rates than their WT or uninjected counterparts. (A) Heart rates were counted in zebrafish larvae under anesthesia at 72 h post−fertilization (hpf). * p ≤ 0.05, as per the two-tailed unpaired t−test, error bars represent SD, and each point represents an individual larva, green represents chd7^+/+, orange represents chd7^+/+ (+/+ n = 24, −/− n = 18). (B) Zebrafish Casper embryos were injected with morpholinos (MOs) at the one−cell stage to knock down RNA expression, and heart rates were counted under anesthesia at 72 hpf. Embryos injected with phox2ba MO had significantly lower heart rates than control MO−injected fish. * p < 0.05, as per one−way ANOVA with a Tukey post−test, error bars represent SD, each point represents an individual larva, green represents control MO, orange represents phox2ba MO, blue represents phox2bb MO n ≥ 9 larvae/group.