FIGURE

Figure S2.

ID

ZDB-FIG-230523-25

Publication

Cevik et al., 2023 - WDR31 displays functional redundancy with GTPase-activating proteins (GAPs) ELMOD and RP2 in regulating IFT complex and recruiting the BBSome to cilium

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Figure S2.

Ciliary localization of WDR31 and ELMOD3 in hTERT-RPE1 cells.

(A) Images showing immunostaining of a ciliary marker ARL13B and DAPI (nucleus) in hTERT-RPE1 cells. NT represents no transfection of hTERT-RPE1 with WDR31 (tagged with cyan fluorescent protein) and ELMOD3 (tagged with red fluorescent protein). Scale bars: 10 μm. (B) Images showing immunostaining of hTERT-RPE1 cells transfected with ELMOD3-CFP (cyan) and also stained for ARL13B (yellow). The magenta channel only shows some background but no localization to the cilium. Green regions in the “merge” picture show co-localization of both ELMOD3 and ARL13B proteins. Scale bars: 3 μm. (C) Fluorescence images displaying hTERT-RPE1 Fluorescence images of immunostaining for ARL13B (yellow) and WDR31-RFP (magenta) in hTERT-RPE1 cells transfected with WDR31-RFP (cyan). The cyan channel shows some background, with no cilium localisation. WDR31 and ARL1B proteins are co-localized in the pink sections of the “merge” image. Scale bars: 3 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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