Fig. 5.

Nalidixic acid prevents Shigella dissemination in zebrafish larvae. All data presented here were collected from S. sonnei 53G- or S. flexneri M90T-infected 2 dpf zebrafish larvae that had either been not treated (NT, green) or had been treated with nalidixic acid (NAL, purple). (A) Representative spreading event in a larva infected with S. sonnei 53G GFP (green), with neutrophils labelled in red. The same larva was imaged at 2, 12 and 24 hpi, while being embedded in low melting agarose for the duration of the experiment. The green line through the larval spine indicates the spreading event. Scale bars: 500 µm. (B) Percentage of spreading events in larvae infected with S. sonnei 53G over the course of 24 hpi. Data were pooled from three independent experiments, with n>10 larvae per condition per experiment. ****P<0.0001. Unpaired two-tailed Student's t-test. (C) Representative spreading event in a larva infected with S. flexneri M90T GFP (green), with neutrophils labelled in red. The same larva was imaged at 2, 12 and 24 hpi, while being embedded in low melting agarose for the duration of the experiment. The green line through the larval spine indicates the spreading event. Scale bars: 500 µm. (D) Percentage of spreading events in larvae infected with S. flexneri M90T over the course of 24 hpi. Data were pooled from three independent experiments, with n>10 larvae per condition per experiment. ns, non-significant. Unpaired two-tailed Student's t-test.