FIGURE

Fig. 5.

ID

ZDB-FIG-230503-6

Publication

Truong et al., 2023 - PRDM1 DNA-binding zinc finger domain is required for normal limb development and is disrupted in split hand/foot malformation

Other Figures

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Fig. 5.

Loss of Prdm1a leads to downregulation of important limb development genes in the pectoral fin. RNA-seq was performed on isolated pectoral fin cells from about 250 WT and prdm1a^?/? embryos at 48 hpf. (A) Lateral and (A?) dorsal view of EGFP-positive pectoral fins from the Tg(Mmu:Prx1-EGFP) zebrafish line at 48 hpf before FACS. Dashed lines indicate where the embryos were dissected prior to FACS. Scale bars: 200 �m. (B) Heat map of top 250 differentially expressed genes (P_adj) between WT and prdm1a^?/? embryos. (C) Volcano plot showing spread of differentially expressed genes in pectoral fins of prdm1a^?/? compared to WT embryos. Light blue dots are significant, differentially expressed genes [?log₁₀(P-value)?1.15]. Purple dots are downregulated, selected genes of interest, whereas yellow dots are upregulated genes. (D) Downregulated genes in prdm1a^?/? embryos were subjected to GO (Panther) pathway enrichment analysis. Yellow arrowheads highlight pathways of interest. A, anterior; ba, branchial arches; DEG, differentially expressed genes; e, eye; P, posterior; pf, pectoral fin; y, yolk.

Expression Data

Gene:	EGFP
Fish:	prdm1a^m805/+; co1026Tg
Anatomical Terms:	head dorsal region pectoral fin pharyngeal arch
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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