FIGURE

Figure 7

ID

ZDB-FIG-230502-8

Publication

Kondo et al., 2023 - The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney

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Figure 7

Loss of Jagged-1a results in a defect in hematopoietic niches

(A and B) Dorsal view of a wild-type or jagged-1a^kz4 kidney.

(C) Transverse section of a jagged-1a^kz4 kidney under a kdrl:Cerulean background. Blue areas outlined by the white dotted line denote the sinusoidal area.

(D) Percentages of sinusoidal area in the wild-type or jagged-1a^kz4 kidney. Error bars, s.d. (n = 4 for each).

(E) Experimental procedure for transplantation assay using wild-type or jagged-1a^kz4 recipients.

(F) Absolute numbers of dsRed⁺ cells in wild-type or jagged-1a^kz4 recipients (mean ± s.e.m; n = 11 [wild-type] or 8 [jagged-1a^kz4]).

(G) Experimental procedure for homing assay using wild-type or jagged-1a^kz4 recipients.

(H) Absolute numbers of BFP⁺ cells in wild-type or jagged-1a^kz4 recipients (mean ± s.e.m; n = 11 for each). AF in A-C denotes auto-fluorescence of renal tubules. ∗p < 0.05; ∗∗p < 0.01. Bars, 200 μm (A and B); 20 μm (C).

Expression Data

Gene:	Cerulean
Fish:	sd24Tg jag1a^kz4/kz4; sd24Tg
Anatomical Term:	kidney sinusoidal blood vessel endothelium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jag1a^kz4/kz4 jag1a^kz4/kz4; sd24Tg
Observed In:	kidney kidney regulation of hematopoietic progenitor cell differentiation sinusoidal blood vessel endothelium
Stage:	Adult

Phenotype Detail

Acknowledgments

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