FIGURE

FIGURE 2

ID

ZDB-FIG-230429-2

Publication

Vedder et al., 2023 - pyHeart4Fish: Chamber-specific heart phenotype quantification of zebrafish in high-content screens

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FIGURE 2

Screening and data analysis workflow. Eggs were collected after 1 h of pair- or group-wise mating before being incubated overnight. At 21 h post-fertilization (hpf), embryos were dechorionated, then at 24 hpf were treated with compounds from the Prestwick drug library. Treatment was stopped at 48 hpf, and fish were mounted in orientation plates to visualize fluorescent hearts and imaged with an ACQUIFER imaging machine. Displayed data types can be quantified using the pyHeart4Fish GUI and result in a graphical output in the form of GIFs and PNGs, as well as data sheets with additional meta and quantitative data. This figure was created with BioRender.com.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol