FIGURE

Fig. 7

ID

ZDB-FIG-230424-74

Publication

Athapaththu et al., 2022 - Pinostrobin ameliorates lipopolysaccharide (LPS)-induced inflammation and endotoxemia by inhibiting LPS binding to the TLR4/MD2 complex

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Pinostrobin reduces LPS-induced macrophage and neutrophil recruitment in zebrafish larvae. Zebrafish larvae at 3 dpf (n = 20) were microinjected with 2 nL of 0.5 mg/mL LPS and immediately treated with pinostrobin (0–20 μM) for 24 h (at 4 dpf). (A) Neutral red staining of macrophages and (B) Sudan black staining for neutrophils were performed. (C) In a parallel experiment, total RNA was isolated, and RT-PCR was performed. The expression of iNOS, COX-2a, IL-12, and TNF-α was measured and normalized compared to that of β-actin expression. ^###, p < 0.001 vs. PBS-microinjected zebrafish larvae; **, p < 0.01 and ***, p < 0.001 vs. LPS-microinjected zebrafish larvae.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Pharmacother.