FIGURE

Fig. 3

ID

ZDB-FIG-230424-68

Publication

Athapaththu et al., 2022 - Pinostrobin ameliorates lipopolysaccharide (LPS)-induced inflammation and endotoxemia by inhibiting LPS binding to the TLR4/MD2 complex

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Fig. 3

Pinostrobin downregulates LPS-stimulated IL-12 and TNF-α production by suppressing their gene expression. RAW 264.7 macrophages were pretreated with pinostrobin (0–20 μM) 2 h before incubation with 300 ng/mL LPS. After 24-h incubation with LPS, (A) IL-12 and (B) TNF-α release was measured in the culture supernatants using ELISA. (C) Total RNA was extracted 6 h after LPS treatment, and RT-PCR was performed. GAPDH was used for normalizing IL-12 and TNF-α expression. The relative density was calculated using ImageJ software. ^###, p < 0.001 vs. untreated cells; *, p < 0.05^, **, p < 0.001, and ***, p < 0.001 vs. LPS-treated cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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