Figure 5

Treatment of rhGH alleviated the hyperactivity-like phenotype of ghrelin^?/? zebrafish. (A) Representative images of zebrafish swimming tracks. (B) Quantification of the spontaneous locomotor distance of zebrafish over 20 min (Mean ± SEM. **P<0.05, ****P<0.0001, NS=no significance, F _{1,26 (interaction)} =6.884, two-way ANOVA). (C) The activity recorded was the mean average swimming distance of adult ghrelin^?/? and adult ghrelin^+/+ zebrafish during 20 min under light-changing condition (first 5 min dark, next 5 min light stimulation, then 5 min dark, and finally 5 min light). (D) The swimming distance of the zebrafish is in the dark (Mean ± SEM. *P<0.05, ****P<0.0001, NS=no significance, F _{1,27 (interaction)} =10.84, two-way ANOVA). (E) The swimming distance of zebrafish under light stimulation (Mean ± SEM. *P<0.05, ****P<0.0001, NS=no significance, F _{1,26 (interaction)} =5.173, two-way ANOVA). (F) The swimming distance of zebrafish in the light (Mean ± SEM.*P<0.05, ****P<0.0001, NS=no significance, F _{1,27 (interaction)} =0.5135, two-way ANOVA). (Ghrelin^+/+ zebrafish injection vehicle, n=8; ghrelin^+/+ zebrafish injection rhGH, n=9; and ghrelin^?/? zebrafish injection vehicle, n=7, ghrelin^?/? zebrafish injection rhGH, n=8).