Fig 2
- ID
- ZDB-FIG-230420-34
- Publication
- Eeza et al., 2023 - Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging
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Chemical shift imaging.
(A) Matrix display of chemical shift imaging spectra. CSI data was recorded with a TR = 3500 ms; TE = 15 ms and slice thickness was 2 mm. Total averages were 256. The resolution obtained was 1.25×1.25×2 mm3. The spectral width used was 10 kHz (13.33 ppm) and 32x32 matrix was reconstructed into 64×64 voxels. Inset: Representative spectra of a single voxel showing residual water (1) and fat resonances (2). The main?(CH2)n?signal in muscles is centered around 1.3 ppm, with side lobes from?(CH2)n?CH3 up-field and -CH2-CH = CH-,?CH2?CH2?COOR extending downfield. (B) CSI voxel intensity thresholding. Signals between 0.80 to 1.25 ppm corresponding to fat were chosen to reconstruct CSI images and they were overlaid with corresponding T2-weighted RARE images using the Bruker CSI Visualisation Tool. Scale bar: 1 mm. |