FIGURE

Figure 1.

ID

ZDB-FIG-230327-11

Publication

Liang et al., 2023 - In vivo DNA methylation editing in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 1.

(a) Domain structure of the dCas9?dnmt7/Tet2 catalytic domain fusion protein. The nuclease inactivating mutations D10A and H840A of Cas9 are indicated. Deactivated Cas9 was fused to the catalytic domain of the zebrafish de novo DNA methyltransferase 7 (Dnmt7CD) or ten-eleven translocation 2 (Tet2CD) using a short Gly4Ser peptide (GS); (b) Illustration of the locations of the three gRNAs designed to target the upstream regions of the dmrt1 coding sequence; (c) Average DNA methylation level of target locus within dmrt1 TSS region (Two-way ANOVA test); (d) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene. The asterisk indicates statistical significance. (* p<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Epigenetics