ZFIN Image: Liang et al., 2023, Figure 1.

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Figure Caption

Figure 1.

(a) Domain structure of the dCas9–dnmt7/Tet2 catalytic domain fusion protein. The nuclease inactivating mutations D10A and H840A of Cas9 are indicated. Deactivated Cas9 was fused to the catalytic domain of the zebrafish de novo DNA methyltransferase 7 (Dnmt7CD) or ten-eleven translocation 2 (Tet2CD) using a short Gly4Ser peptide (GS); (b) Illustration of the locations of the three gRNAs designed to target the upstream regions of the dmrt1 coding sequence; (c) Average DNA methylation level of target locus within dmrt1 TSS region (Two-way ANOVA test); (d) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene. The asterisk indicates statistical significance. (* p<0.05).

Acknowledgments

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