Fig. 6
- ID
- ZDB-FIG-230322-41
- Publication
- Tyrkalska et al., 2022 - Silica crystals activate toll-like receptors and inflammasomes to promote local and systemic immune responses in zebrafish
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Fig. 6. Silica crystals signal through the Nlrp3/Caspa/Gsdme inflammasome in zebrafish. Tg(lyz:DsRED2) (A, D, E, H), Tg(mfap4:mCherry) (B, F, I) and Tg(NFkB-RE:eGFP) (C, G, J) 1–8 cell stage embryos were microinjected with control, gsdmea/b (A-D), nlrp3 (E-G) or il1b (H-J) crRNA/Cas9 complexes. At 2 dpf, SiO2 crystals or PBS were injected in the larval hindbrain ventricle (HBV) in the presence of either DMSO or the caspase-1 inhibitor VX-765 (VX) (D). Neutrophil (A, D, E, H) and macrophage (B, F, I) recruitment and number, and Nfkb activity (C, G, J) were analyzed at 6, 12 and 24 hpi by fluorescence microscopy. Each dot represents one individual and the mean ± S.E.M. for each group is also shown. P values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant, *p ≤ p0.05, **p ≤ 0.01, ***p ≤ 0.001. |