Figure 1.
- ID
- ZDB-FIG-230315-33
- Publication
- Liao et al., 2023 - Vitamin D influences gut microbiota and acetate production in zebrafish (Danio rerio) to promote intestinal immunity against invading pathogens
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VD strengthened the intestinal health and antimicrobial responses in zebrafish. (a) WT and cyp2r1-/- zebrafish at 3 mpf were i.p. injected with 107 CFU E. tarda per fish. The survival rate of each genotype was recorded as survival rate curves (n = 8/genotype). (b) The gene expression of zfbd1, zfbd2, zfbd3 in the intestine of WT and cyp2r1-/- zebrafish was analyzed by qRT-PCR. (c-d) Zebrafish at 2 mpf were fed with the diet containing 0 or 800 IU/kg VD3 for 4 weeks. Thereafter, zebrafish were i.p. injected with 106 CFU E. tarda per fish (n = 10/group), and the survival rate of each genotype was recorded (c). Furthermore, the gene expression of zfbd1, zfbd2, zfbd3 in zebrafish intestine was analyzed (d). (e-f) The gene expression of il22 and rorc (e), as well as the protein level of IL-22 (f) in the intestine of WT and cyp2r1-/- zebrafish was assessed. In addition, (g-h) the gene expression of il22 and rorc (g), and the protein level of IL-22 (h) in the zebrafish fed non-VD or VD-containing diet was analyzed. The images are representative of the results from western blots. *p < 0.05, **p < 0.01, ***p < 0.001. WT, wild type; zfbd, zebrafish β-defensin. See also Figures S1. |