FIGURE

Figure S5.

ID

ZDB-FIG-230308-16

Publication

Mi et al., 2023 - Efficient knock-in method enabling lineage tracing in zebrafish

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Figure S5.

Lineage tracing and cell labelling of <italic toggle='yes'>nkx6.1</italic><sup>+</sup> cells in the CNS and pancreas.

(A, B, C) Representative confocal images of nkx6.1-expressing and lineage-traced cells in the CNS (A, A’, A’’, B, B’, B’’) and pancreas (C, C’, C’’) in zebrafish larvae at 6 dpf. (C) The white dashed lines outline the whole pancreata (C, C’, C’’). (D, E, F) Representative confocal images of nkx6.1-expressing cells in the intrapancreatic duct in 6 dpf zebrafish larvae. (D, E, F) The pancreata are outlined by white dashed lines (D, E, F). (E, F) Arrowhead points to the principal islet depicted by Insulin staining displayed in grey (E, F). (G, H, I) Representative confocal images of pancreata showing the contribution of nkx6.1-traced cells in the pancreas with acinar cells labelled with ptf1a:EGFP (G, G’, G’’, G’’’) or ela3l:H2BGFP (H, H’, H’’, H’’’) with magnifications for improved visualization (I, I’, I’’, I’’’), in 6 dpf zebrafish larvae. Low laser power was used to not visualize mNeonGreen while observing EGFP/H2BGFP. The white dashed lines outline the whole pancreata. (A, B, C, D, E, F, G, H) Scale bars = 200 μm (A, B, C) or 80 μm (D, E, F, G, H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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