Fig. 1

Intubation of an adult fish on an inverted microscope. (A-D) Photographs of long-term imaging of an intubated adult zebrafish inside a custom 3D-printed chamber. (A) Overview photograph of an intubation rig set up on an inverted microscope. (B) Photograph of the intubation/imaging chamber set up on a microscope stage, showing inflow tube, dual outflow tubes, overflow tube, water sensor and sponge holding fish in place. (C) Close-up photograph of the chamber with an intubated fish, with inflow tube, dual outflow tubes and water sensor noted. (D) Higher magnification image of the intubated fish. (E,F) 3D renderings of intubation chambers designed to fit on (E) a Tokai Hit heated stage (model INUB-TIZB) or (F) in a 96-well plate holder on a Tokei Hit heated stage (model STZF-TIZWX-SET), or any other stage designed to hold 96-well plates.